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Sangon Biotech protein a 302 affinity chromatography
Protein A 302 Affinity Chromatography, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+a+affinity+chromatography/302+a+affinity+chromatography+protein/pm41935577-167-10-15
Average 86 stars, based on 1 article reviews
protein a 302 affinity chromatography - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Recombinant:

Article Title: Identification of the atypical cadherin FAT1 as a novel glypican-3 interacting protein in liver cancer cells
Article Snippet: .. Recombinant hFc-tagged protein was purified by using protein A affinity chromatography (Sangon Biotech). .. Cells were cultured in flask or plate for indicated conditions, and lysed with RIPA buffer (P0013K, Beyotime) containing cocktail protease and phosphatase inhibitors (P1008, Beyotime).

Purification:

Article Title: Identification of the atypical cadherin FAT1 as a novel glypican-3 interacting protein in liver cancer cells
Article Snippet: .. Recombinant hFc-tagged protein was purified by using protein A affinity chromatography (Sangon Biotech). .. Cells were cultured in flask or plate for indicated conditions, and lysed with RIPA buffer (P0013K, Beyotime) containing cocktail protease and phosphatase inhibitors (P1008, Beyotime).

Article Title: Strengthening of antitumor effects in breast cancer from a novel B7-H4- and CD3-targeting bispecific antibody by an oncolytic virus
Article Snippet: The transfected cells were cultured in SMM 293-TII Expression Medium (cat. no. M293TII; Sino Biological Inc., Beijing, China). .. The expressed protein was secreted into the cell culture medium and purified by protein A affinity chromatography (cat. no. C600952-0501; Sangon Biotech Co., Ltd; Shanghai, China). ..

Affinity Chromatography:

Article Title: Identification of the atypical cadherin FAT1 as a novel glypican-3 interacting protein in liver cancer cells
Article Snippet: .. Recombinant hFc-tagged protein was purified by using protein A affinity chromatography (Sangon Biotech). .. Cells were cultured in flask or plate for indicated conditions, and lysed with RIPA buffer (P0013K, Beyotime) containing cocktail protease and phosphatase inhibitors (P1008, Beyotime).

Article Title: Strengthening of antitumor effects in breast cancer from a novel B7-H4- and CD3-targeting bispecific antibody by an oncolytic virus
Article Snippet: The transfected cells were cultured in SMM 293-TII Expression Medium (cat. no. M293TII; Sino Biological Inc., Beijing, China). .. The expressed protein was secreted into the cell culture medium and purified by protein A affinity chromatography (cat. no. C600952-0501; Sangon Biotech Co., Ltd; Shanghai, China). ..

Cell Culture:

Article Title: Strengthening of antitumor effects in breast cancer from a novel B7-H4- and CD3-targeting bispecific antibody by an oncolytic virus
Article Snippet: The transfected cells were cultured in SMM 293-TII Expression Medium (cat. no. M293TII; Sino Biological Inc., Beijing, China). .. The expressed protein was secreted into the cell culture medium and purified by protein A affinity chromatography (cat. no. C600952-0501; Sangon Biotech Co., Ltd; Shanghai, China). ..

other:

Article Title: Construction and Application of a Canine SLAM Receptor-Based System from Vero Cell Line to Virus Isolation and Parallel Antibody Screening
Article Snippet: The extracellular domain of canine signaling lymphocyte activation molecule (SLAM) (NP_001003084.1, amino acids 27–237) was fused with human IgG1-Fc and cloned into the mammalian expression vector pFUSE-hIgG1-Fc2 (InvivoGen, San Diego, CA, USA) to generate SLAM-hFc.



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